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Informant: Sibylle Gabriel
Informant: Sibylle Gabriel
Starmail - 30. Jul, 22:14
30
Jul
2006
Modest increase in temperature affects ODC activity in L929 cells: low-level radiofrequency radiation does not
Radiat Environ Biophys. 2006 Jul 19; [Epub ahead of print]
* Hoyto A, * Sihvonen AP, * Alhonen L, * Juutilainen J, * Naarala J.
Department of Environmental Sciences, University of Kuopio, P.O. Box 1627, Kuopio, 70211, Finland, Anne.Hoyto@uku.fi.
The effects of low-level radiofrequency (RF) radiation and elevated temperature on ornithine decarboxylase (ODC) activity were investigated in murine L929 fibroblasts. The cells were exposed at 900 MHz either to a pulse-modulated (pulse frequency 217 Hz; GSM-type modulation) or a continuous wave signal at specific absorption rate (SAR) levels of 0.2 W kg(-1) (0.1-0.3 W kg(-1)) and 0.4 W kg(-1) (0.3-0.5 W kg(-1)) for 2, 8, or 24 h. RF radiation did not affect cellular ODC activity. However, a slight increase in temperature (0.8-0.9 degrees C) in the exposure system lead to decreased ODC activity in cell cultures. This was verified by tests in which cells were exposed to different temperatures in incubators. The results show that ODC activity is sensitive to small temperature differences in cell cultures. Hence, a precise temperature control in cellular ODC activity studies is needed.
PMID: 16850337 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16850337&itool=pubmed_DocSum
* Hoyto A, * Sihvonen AP, * Alhonen L, * Juutilainen J, * Naarala J.
Department of Environmental Sciences, University of Kuopio, P.O. Box 1627, Kuopio, 70211, Finland, Anne.Hoyto@uku.fi.
The effects of low-level radiofrequency (RF) radiation and elevated temperature on ornithine decarboxylase (ODC) activity were investigated in murine L929 fibroblasts. The cells were exposed at 900 MHz either to a pulse-modulated (pulse frequency 217 Hz; GSM-type modulation) or a continuous wave signal at specific absorption rate (SAR) levels of 0.2 W kg(-1) (0.1-0.3 W kg(-1)) and 0.4 W kg(-1) (0.3-0.5 W kg(-1)) for 2, 8, or 24 h. RF radiation did not affect cellular ODC activity. However, a slight increase in temperature (0.8-0.9 degrees C) in the exposure system lead to decreased ODC activity in cell cultures. This was verified by tests in which cells were exposed to different temperatures in incubators. The results show that ODC activity is sensitive to small temperature differences in cell cultures. Hence, a precise temperature control in cellular ODC activity studies is needed.
PMID: 16850337 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16850337&itool=pubmed_DocSum
Starmail - 30. Jul, 18:17
Evidence for a specific microwave radiation effect on the green fluorescent protein
Biophys J. 2006 Aug 1;91(4):1413-23. Epub 2006 May 26.
Copty AB, Neve-Oz Y, Barak I, Golosovsky M, Davidov D.
The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, Israel.
We have compared the effect of microwave irradiation and of conventional heating on the fluorescence of solution-based green fluorescent protein. A specialized near-field 8.5 GHz microwave applicator operating at 250 mW input microwave power was used. The solution temperature, the intensity, and the spectrum of the green fluorescent protein fluorescence 1), under microwave irradiation and 2), under conventional heating, were measured. In both cases the fluorescence intensity decreases and the spectrum becomes red-shifted. Although the microwave irradiation heats the solution, the microwave-induced changes in fluorescence cannot be explained by heating alone. Several possible scenarios are discussed.
PMID: 16731554 [PubMed - in process]
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=retrieve&db=pubmed&list_uids=16731554&dopt=Abstract
Copty AB, Neve-Oz Y, Barak I, Golosovsky M, Davidov D.
The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, Israel.
We have compared the effect of microwave irradiation and of conventional heating on the fluorescence of solution-based green fluorescent protein. A specialized near-field 8.5 GHz microwave applicator operating at 250 mW input microwave power was used. The solution temperature, the intensity, and the spectrum of the green fluorescent protein fluorescence 1), under microwave irradiation and 2), under conventional heating, were measured. In both cases the fluorescence intensity decreases and the spectrum becomes red-shifted. Although the microwave irradiation heats the solution, the microwave-induced changes in fluorescence cannot be explained by heating alone. Several possible scenarios are discussed.
PMID: 16731554 [PubMed - in process]
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=retrieve&db=pubmed&list_uids=16731554&dopt=Abstract
Starmail - 30. Jul, 18:10
