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Dear Hans: This is probably quite an important paper because it explains that ELF's can inhibit or stimulate depending on condition of the cells.

I have been searching and searching for some sort of info regarding how it is determined strength of field to be applied in regard to healing a broken bone and also how they know to wait a certain amount of time before having person apply the EMF stimulator (or whatever the name of equipment is) after initial use and so on.

Obviously in the case of a break that isn't healing, the medical profession most likely applies past experience and "educated guesses" followed by X-rays to determine need for additional EMF stimulation.

This is the first time I have ever seen a reference that includes both inhibition of cell growth and stimulation of cell growth.

Take care - Joanne

Joanne C. Mueller Guinea Pigs R Us
731 - 123rd Avenue N.W. Minneapolis, Minnesota 55448-2127 USA Phone: 763-755-6114 Email: jcmpelican@aol.com [3-17-06]


Betreff: ELF...effects on cell growth re inhibit...may stimulate or inhib depend on cells
Von: JCMPelican@aol.com
Datum: Sat, 18 Mar 2006 01:31:02 EST

Mutat Res. 2001 Jun 27;493(1-2):55-66.
ELF magnetic field affects proliferation of SPD8/V79 Chinese hamster cells but does not interact with intrachromosomal recombination.

Olsson G, Belyaev IY, Helleday T, Harms-Ringdahl M.

Department of Genetic and Cellular Toxicology, Stockholm University, Svante Arrhenius vag 16-18, S-106 91 Stockholm, Sweden. gunilla.olsson@genetics.su.se

Extremely low-frequency (ELF) magnetic fields have previously been shown to affect conformation of chromatin, cell proliferation, and calcium metabolism. Possible mutagenic and carcinogenic effects of ELF have also been discussed and tested. In this study, intrachromosomal recombination in the hprt gene after exposure to ELF magnetic field was investigated using the SPD8 recombination assay. SPD8 cells, derived from V79 Chinese hamster cells were exposed to ELF at a specific combination of static and ELF magnetic fields, that has been proven to have effects on chromatin conformation in several cell types. The genotoxic agent camptothecin (CPT) was used either as a positive control or simultaneously with ELF. We also analysed the effect of ELF and CPT on chromatin conformation with the anomalous viscosity time dependence (AVTD) technique, cell growth kinetics, and cell survival with clonogenic assay. DNA fragmentation was analysed by pulsed field gel electrophoresis (PFGE). ELF did not induce recombination alone, neither did ELF modify the recombinogenic effect of CPT. Although, there was no effect on cell survival in response to ELF exposure, inhibition of cell growth was observed. On the other hand, ELF exposure partly counteracted the growth inhibition seen with CPT. The data suggest that ELF exposure may stimulate or inhibit cell growth depending on the state of the cells. Although, ELF did not induce recombination, a weak but statistically significant DNA fragmentation comparable with CPT-induced fragmentation was observed with PFGE 48h after exposure to ELF.

PMID: 11516715 [PubMed - indexed for MEDLINE]

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