Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells
Study
Mouse embryonic stem (ES) cells were used as an experimental model to study the effects of intermittent EMF exposures (5min on/30 minutes off). ELF-EMF exposure (2mT 50Hz with worst-case distortions for supply) to ES-derived neural cells significantly affected transcript levels of the apoptosis-related bcl-2, bax, and cell cycle regulatory "growth arrest DNA damage inducible" GADD45 genes, whereas mRNA levels of neural-specific genes were not affected. RF-EMF exposure (GSM-1800 simulated exposure at time-averaged SAR of 1.5 W/kg) of neural progenitor cells resulted in down-regulation of neural-specific Nurr1 and in up-regulation of bax and GADD45 mRNA levels. Short-term RF-EMF exposure for 6 h, but not for 48 h, resulted in a low and transient increase of DNA double-strand breaks.
Bibliographic information: Teodora Nikolova, Jaroslaw Czyz, Alexandra Rolletschek, Przemyslaw Blyszczuk, Jörg Fuchs, Gabriele Jovtchev, Jürgen Schuderer, Niels Kuster, and Anna M. Wobus, in The FASEB Journal, fj.04-3549fje, published online 22 August, 2005
http://www.fasebj.org/cgi/content/abstract/04-3549fjev1
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16116041&query_hl=2
From FGF-Infoline 02.09.2005
Mouse embryonic stem (ES) cells were used as an experimental model to study the effects of intermittent EMF exposures (5min on/30 minutes off). ELF-EMF exposure (2mT 50Hz with worst-case distortions for supply) to ES-derived neural cells significantly affected transcript levels of the apoptosis-related bcl-2, bax, and cell cycle regulatory "growth arrest DNA damage inducible" GADD45 genes, whereas mRNA levels of neural-specific genes were not affected. RF-EMF exposure (GSM-1800 simulated exposure at time-averaged SAR of 1.5 W/kg) of neural progenitor cells resulted in down-regulation of neural-specific Nurr1 and in up-regulation of bax and GADD45 mRNA levels. Short-term RF-EMF exposure for 6 h, but not for 48 h, resulted in a low and transient increase of DNA double-strand breaks.
Bibliographic information: Teodora Nikolova, Jaroslaw Czyz, Alexandra Rolletschek, Przemyslaw Blyszczuk, Jörg Fuchs, Gabriele Jovtchev, Jürgen Schuderer, Niels Kuster, and Anna M. Wobus, in The FASEB Journal, fj.04-3549fje, published online 22 August, 2005
http://www.fasebj.org/cgi/content/abstract/04-3549fjev1
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16116041&query_hl=2
From FGF-Infoline 02.09.2005
Starmail - 3. Sep, 18:35
